| Dyn | Manchester | 1998 |
| E-mu Systems | Scotts Valley | 1971 |
| E. Remington and Sons | Estados Unidos | 1816 |
| EA Sports | Burnaby | 1991 |
| EarthLink | Atlanta, Pasadena, Atwater Village | 1994 |
| Eastman Chemical Company | Kingsport | 1920 |
| Eaton Corporation | Cleveland | 1911 |
| EBay | São José | 3 de setembro de 1995 |
| Eclipse Comics | Forestville, Staten Island | 1977 |
| Edison Manufacturing Company | Nova Iorque | 1889 |
| Einstein Bros. Bagels | Lakewood | 1995 |
| Electro-Motive Diesel | La Grange | 1922 |
| Elevance Health | Indianápolis | 2004 |
| EMC Corporation | Hopkinton | agosto 1979 |
| EnterpriseDB | Bedford | março 2004 |
| EPAM Systems | Newtown, Pensilvânia | 1993 |
| Epicor | Austin, Irvine | 1972 |
| Epyx | São Francisco | 1978 |
| Equinix | Redwood City | 22 de junho de 1998 |
| ESPN+ | Estados Unidos | 12 de abril de 2018 |
| ESPN, LLC | Bristol | 7 de setembro de 1979 |
| Estée Lauder Companies | Neuilly-sur-Seine, Nova Iorque | 1946 |
| Estúdios Keystone | Edendale | 1912 |
| EVOLVE Wrestling | Filadélfia | 2009 |
| Exidy | Sunnyvale | 1974 |
| Expeditors International | Seattle | 1979 |
| Extreme Championship Wrestling | Filadélfia | 25 de abril de 1992 |
| Exxon | Las Colinas | 1 de janeiro de 1973 |
| ExxonMobil | Spring | 5 de agosto de 1882 |
| Fageol | Oakland | 1916 |
| Fannie Mae | Noroeste de Washington, D.C. | 1938 |
| Fantasy Flight Games | Roseville | 1995 |
| Fastly | São Francisco | 2011 |
| FedEx | Memphis | 1971 |
| FedEx Office | Dallas | 1970 |
| FEI Company | Hillsboro | 1971 |
| Fenway Sports Group | Boston | 2001 |
| Fidelity Investments | Boston | 1946 |
| Firebase | São Francisco | 2010 |
| Firefly Aerospace | Cedar Park | 2014 |
| Firehouse Subs | — | 1994 |
| First Citizens BancShares | Raleigh | 7 de agosto de 1986 |
| First Data | Atlanta | 1969 |
| Florsheim Shoes | Glendale | 1892 |
| Fluor Corporation | Irving | 1890 |
| FMC Corporation | Mellon Bank Center | 1883 |
| FMC Technologies | Houston | 2000 |
| Ford Aerospace | Milpitas | 1956 |